Apoa2 humanized transgenic mouse – line beta

The mice are hemizygous for the human apolipoprotein A2 (ApoA2) gene expressed under the control of the homologous promoter (-911/+2045). They are humanized because they are deficient in mouse ApoAII following backcrosses with APOA2 Knockout mice (KO-ApoA2 described in ref. 1). KO-ApoA2 mice have been fully backcrossed into the C57BL/6 background before mating with human ApoAII transgenic mice.
ApoAII is expressed only in liver. Its plasma concentration is 20-30 mg/dl with chow diet (physiological concentration in humans), and increases up to 50 mg/dl with a high fat diet.
Mice present with normal plasma HDL levels and normal lipoprotein profile in the fed and fasted states (2).

Interest / Relevance: ApoAII is the second most important protein of HDL (High Density Lipoproteins) after ApoAI, and ensures HDL structural stability.
Human ApoAII promotes cholesterol efflux mediated by ABCA1 (3).
Useful for studying the role of human Apo AII expressed at physiological levels and the influence of dietary and hormonal treatments (due to the presence of the homologous promoter).

Keywords: ApoA2

Publications:

11- Dramatically decreased high density lipoprotein cholesterol, increased remnant clearance, and insulin hypersensitivity in apolipoprotein A-II knockout mice suggest a complex role for apolipoprotein A-II in atherosclerosis susceptibility. Weng W. and Breslow JL., Proc Natl Acad Sci U S A 1996, 93:14788-94.

2- Apolipoprotein A-II/A-I ratio is a key determinant in vivo of HDL concentration and formation of pre-beta HDL containing apolipoprotein A-II. Pastier D. et al., Biochemistry 2001, 40:12243-53.

3- Opposite effects of plasma from human apolipoprotein A-II transgenic mice on cholesterol efflux from J774 macrophages and Fu5AH hepatoma cells. Fournier N. et al., Arterioscler Thromb Vasc Biol 2002, 22:638-43.

Reference:

RT00403