Back to results

Meln (her alpha) cell line

MELN were generated by transfection of breasr cancer MCF-7 cells with the ERE-betaGlobin-Luciferase-SV40-Neomycin plasmid. rnThe plasmid ERE-betaGlob-Luc-SVNeo contains a luciferase gene driven by estrogen receptor responsive element (ERE) in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. rn
Interest / Relevance: These cells enable to measure the hER alpha estrogenic activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics). rnThese cells enable to measure the hER alpha estrogenic activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.rn
Keywords: Estrogen receptor alpha, luciferase, pharmaceutical and environmental estrogens
Scientist's name: Patrick BALAGUER
Patrick Balaguer
Publications:
Balaguer P Boussioux AM Demirpence E Nicolas JC. Reporter cell lines are useful tools for monitoring biological activity of nuclear receptor ligands. Luminescence. 2001 16:1 53-8. rnFenet H Gomez E Pillon A Rosain D Nicolas JC Casellas C Balaguer P. Estrogenic activity in water and sediments of a French river: contribution of alkylphenols. Arch Environ Contam Toxicol. 2003 44: 1-6. rn

Reference:

RT00583

Business Developper
contact
Inserm Transfert
Research Tools
Rare disease: No
Last update: 08/07/2024

You might also be interested in

RT00586

Ualh har cell line

UALH were generated by transfection of osteocarcinoma cancer U2OS cells with the […]

Read more
RT00582

Palm cell line

PALM were generated by co-transfection of cervical cancer PC3 cells with the […]

Read more
RT00623

Hrln (hrar alpha) cell line

HRLN were generated by transfection of cervical cancer HeLa cells with the […]

Read more
RT00626

Ug5ln gal4-hmr cell line

UG5LN were generated by transfection of osteocarcinoma cancer U2OS cells with the […]

Read more
RT00625

Ug5ln cell line

UG5LN were generated by transfection of osteocarcinoma cancer U20S cells with the […]

Read more
RT00624

Ualh cell line

UALH were generated by transfection of osteocarcinoma cancer U2OS cells with the […]

Read more
RT00558

Hg5ln gal4-hppar alpha cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the […]

Read more
RT00559

Hg5ln gal4-hppar gamma cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the […]

Read more
RT00569

Hg5ln gal4-hppar beta/delta cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the […]

Read more
RT00585

Hg5ln rtr alpha cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the […]

Read more
RT00588

Hg5ln hpxr cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the […]

Read more
RT00589

Hmln (hgr) cell line

HMLN were generated by transfection of cervical cancer HeLa cells with the MMTV- […]

Read more