Palm cell line

PALM were generated by co-transfection of cervical cancer PC3 cells with the MMTV-Luciferase-SV40-Neomycin and the pSG5-human AR-puromycin plasmids. The plasmid MMTV-Luc-SVNeo contains a luciferase gene driven by the mouse mammary tumor virus promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. The pSG5-puro plasmid pSG5-human AR-puromycin enables to express the human androgen receptor (hAR). Puromycin N-acetyl transferase selection marker expression confers resistance to puromycin.

Interest / Relevance: These cells enable to measure the hAR activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics).
These cells enable to measure the hAR activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.

R1881 EC50 0,05 nM
Keywords: hAR, luciferase, pharmaceutical and environmental androgens
Scientist's name: Patrick BALAGUER
Patrick Balaguer

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