Ug5ln gal4-hmr cell line

UG5LN were generated by transfection of osteocarcinoma cancer U2OS cells with the (GAL4RE)5-betaGlobin-Luciferase-SV40-Neomycin plasmid. UG5LN GAL4-hMR cells were generated by transfection of UG5LN cells with the pSG5-puro plasmid pSG5-GAL4-human MR-puromycin. The plasmid (GAL4RE)5-betaGlob-Luc-SVNeo contains a luciferase gene driven by a pentamer of yeast activator GAL4 binding sites in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. The pSG5-puro plasmid pSG5-human GAL4-hMR-puromycin enables to express the DNA binding domain of the yeast activator GAL4 (GAL4 DBD) followed by the ligand binding domain of the human mineralocorticoid receptor (hMR LBD). Puromycin N-acetyl transferase selection marker expression confers resistance to puromycin.

Interest / Relevance: These cells enable to measure the hMR (full and partial agonism, antagonism) of pharmaceuticals, environmental compounds
These cells enable to measure the hMR activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.

aldosterone EC50 0.6 nM
Keywords: hMR, luciferase, metabolism, clearance, colon and liver cancer, pharmaceutical and environmental chemicals
Scientist's name: Patrick BALAGUER

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