Hmln (hgr) cell line

HMLN were generated by transfection of cervical cancer HeLa cells with the MMTV- -Luciferase-SV40-Neomycin plasmid. HMLN hGR alpha cells were generated by transfection of HMLN cells with the plasmid pSG5-human GR-puromycin. The plasmid MMTV-Luc-SVNeo contains a luciferase gene driven by the mouse mammary tumor virus promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. The pSG5-puro plasmid pSG5-human GR-puromycin enables to express the human glucocorticoid receptor (hGR). Puromycin N-acetyl transferase selection marker expression confers resistance to puromycin.

Interest / Relevance: These cells enable to measure the hGR activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics).
These cells enable to measure the hGR activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.
Keywords: hGR, luciferase, pharmaceutical and environmental glucocorticoids
Scientist's name: Patrick BALAGUER
Patrick Balaguer

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