HMLN were generated by transfection of cervical cancer HeLa cells with the MMTV- -Luciferase-SV40-Neomycin plasmid.
HMLN hGR alpha cells were generated by transfection of HMLN cells with the plasmid pSG5-human GR-puromycin.
The plasmid MMTV-Luc-SVNeo contains a luciferase gene driven by the mouse mammary tumor virus promoter and a neomycin phosphotransferase gene under the control of SV40 promoter.
The pSG5-puro plasmid pSG5-human GR-puromycin enables to express the human glucocorticoid receptor (hGR). Puromycin N-acetyl transferase selection marker expression confers resistance to puromycin.