HMLN were generated by transfection of cervical cancer HeLa cells with the MMTV- -Luciferase-SV40-Neomycin plasmid. rnHMLN hGR alpha cells were generated by transfection of HMLN cells with the plasmid pSG5-human GR-puromycin. rnThe plasmid MMTV-Luc-SVNeo contains a luciferase gene driven by the mouse mammary tumor virus promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. rnThe pSG5-puro plasmid pSG5-human GR-puromycin enables to express the human glucocorticoid receptor (hGR). Puromycin N-acetyl transferase selection marker expression confers resistance to puromycin. rn