UG5LN were generated by transfection of osteocarcinoma cancer U20S cells with the (GAL4RE)5-betaGlobin-Luciferase-SV40-Neomycin plasmid. rnThe plasmid (GAL4RE)5-betaGlob-Luc-SVNeo contains a luciferase gene driven by a pentamer of yeast activator GAL4 binding sites in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. rn