Rbl-2h3 rat mast cell line : in vitro degranulation assay for evaluation of ige-reactivity towards environmental allergens in allergic patient serum

Rat mast cell line (RBL-2H3) transfected with the cDNAs encoding the three subunits (alpha, beta, gamma) of the human high affinity IgE receptor. The transfected cell line binds human IgE, which does not bind to rodent receptor. It enablesto perform degranulation assays with serum from allergic patients for its reactivity towards environmental allergens.

Interest / Relevance: 1. Evaluation of patient serum for reactivity towards environmental allergen.
2. Evaluation of adverse allergic effects in clinical studies or trials.

Keywords: allergy , mast cell , human IgE

Scientist's name: Ulrich BLANK

Publications:

F. Marchand, S. Mecheri, L. Guilloux, B. Iannascoli, A. Weyer. and U. Blank (2003). Human serum IgE-mediated mast cell degranulation shows poor correlation to allergen-specific IgE content. Allergy 58 : 1037-1043.

M. Bodinier, C. Brossard, S. Triballeau, M. Morisset, C. Guérin-Marchand, F. Pineau, P. de Coppet, DA. Moneret-Vautrin, U. Blank and S. Denery-Papini. Evaluation of an in vitro mast cell degranulation test in the context of food allergy to wheat. Int Arch Allergy Immunol 146(4):307-320

Reference:

RT00394

Type of research tool: Cell Lines

Therapy area(1): Others

Uses(1): Academic Research

Uses(2): Proof of concept in vitro

Uses(3): Industry Research (screening, tox.studies, bioreactor, ...)

Uses(4): Diagnostic

Inserm Transfert
Research Tools

E-mail

Organism: Rat (Lewis)

Tissue: Mucosal mast cell line

Morphology: NA

Passage Number: Unknown

Subculture Routine: NA

Culture Medium: DMEM with 10% fetal calf serum

Growth Properties: Adherent

Karyotype: NA, In vitro human mast cell degranulation assay

Rare disease: No

Last update: 14/11/2024

Rbl-2h3 rat mast cell line : in vitro degranulation assay for evaluation of ige-reactivity towards environmental allergens in allergic patient serum

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