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Cpt1 expressing yeast strains

Saccharomyces cerevisiae expressing under the GAL promoter either the wild-type rat liver CPT1, mutated rat liver CPT1 or chimeric CPT. CPT1A activity is inhibited by malonyl-CoA, its allosteric inhibitor.

Interest / Relevance: Allow to have isolated mitochondria with wild-type rat liver CPT1 (active and sensitive to malonyl-CoA inhibition) or mutated CPT1 (for example, active but insensitive to malonyl-CoA inhibition). These mitochondria could be used as biological material for screening chemical molecules able to modulate CPT1 activity and malonyl-CoA sensitivity.

Scientist's name: Carina PRIP-BUUS

Reference:

RT00005

Type of research tool: Cell Lines

Uses(1): Academic Research

Uses(2): Proof of concept in vitro

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Organism: Saccharomyces cerevisiae

Tissue: Unicellular

Morphology: Not relevant

Passage Number: Not relevant

Subculture Routine: Not relevant

Culture Medium: Minimal selective medium. Induction of protein expression by addition of galactose

Growth Properties: Not relevant

Karyotype: Not relevant

Rare disease:

Last update: 23/05/2023

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