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Three-dimensional multicellular spheroid cultures

When culture in suspension many mammalian cell types can aggregate into 3D-spheroids. These multicellular cultures mimic real tissues or tumours and are useful models for therapeutic screening.
Interest / Relevance: Intermediate assay between in cellulo and in vivo cultures; in some extent predictive for in vivo results.
Test useful for:
- Proliferation assays
- Cell-cell interaction analysis
- Drug availability (clearance, half-life, ...)
- Gene profiling

Avantages: long term cultures, daily tracing of spheroid size.
Keywords: proliferation , tumour , cell toxicity , 3d-cultures , cell-cell interactions
Scientist's name: Annie MOLLA
Publications:
Hoang TM, Favier B, Valette A, Barette C, Nguyen CH, Lafanechère L, Grierson DS, Dimitrov S, Molla A.
Benzo[e]pyridoindoles, novel inhibitors of the aurora kinases.
Cell Cycle. 2009 Mar 1;8(5):765-72.

Reference:

RT00396

Business Developper
contact
Inserm Transfert
Research Tools
Accessible for: Fee-for-service research, Collaboration, Training / Know-How License
Equipment available: Cell culture facilities/ microscopy
Samples/Models available: Many cell types are eligible.Requirement: aggregating cells.Examples: NSCLC H358, HCT-116, ...
Rare disease: No
Last update: 11/09/2024

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