Ht29-mtx cells : intestinal model of goblet cells

HT29MTX10-6, HT29MTX10-5 and HT29MTX10-4 cell populations were isolated from the colon cancer cell line by stepwise adaptation to 10-6 M and 10-5 M and 10-4 M Methotrexate (MTX), respectively. HT29MTX cells form a homogeneous population of polarized goblet cells that secrete mucins of gastric immunoreactivity. A mucus gel becomes visible on the surface of the cell layer at postconfluency. These cells maintain their ability to differentiate under normal culture conditions (without MTX). This cell line is available through CelluloNet in Lyon

Interest / Relevance: Polarized cells
Mucus gel on the cell surface
Provide tumor in nude mice (subcutaneous injection)
Growth on tissue culture treated plastic and Transwell membrane
study on proteins linked to goblet cell phenotype, interactions with pathogens, drug transport, drug resistance, co-culture with enterocyte cells
Scientist's name: Thecla LESUFFLEUR

Reference:

RT00309

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Thecla LESUFFLEUR
Inserm Transfert
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Organism: human
Tissue: colon tumor
Morphology: adherente, monolayer of polarized goblet cells with an apical brush border
Passage Number: 12 to 18
Subculture Routine: the cells are passaged every week using 0,05% Trypsin / EDTA and seeded at 2,4x104 cells per cm2
Culture Medium: DMEM Glutamax (4.5 g/L glucose) supplemented with 10% of inactivated FBS.The medium is changed daily from the second day after seeding on (5ml/25cm2)
Growth Properties: doubling-time : 28 hours
Karyotype: aneuploid
Last update: 05/12/2019

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