HT29MTX10-6, HT29MTX10-5 and HT29MTX10-4 cell populations were isolated from the colon cancer cell line by stepwise adaptation to 10-6 M and 10-5 M and 10-4 M Methotrexate (MTX), respectively. HT29MTX cells form a homogeneous population of polarized goblet cells that secrete mucins of gastric immunoreactivity. A mucus gel becomes visible on the surface of the cell layer at postconfluency. These cells maintain their ability to differentiate under normal culture conditions (without MTX).
This cell line is available through CelluloNet in Lyon