Transgenic mice with human ucp3 moderate overexpression show resistance to high fat-induced obesity.

Uncoupling protein (UCP) 3 is a mitochondrial inner membrane protein expressed predominantly in glycolytic skeletal muscles.Transgenic mice were created with a 16 kb region encompassing the human UCP3 gene. Changes in body weight, adiposity and glucose or insulin tolerance were assessed in mice fed chow and high-fat diets. Mitochondrial uncoupling was investigated on permeabilised muscle fibres. Indirect calorimetry was used to determine whole-body energy expenditure and substrate utilisation.

Interest / Relevance: Metabolic analyses
Energy expenditure
Keywords: Obesity , uncoupling protein , glucose tolerance , skeletal muscle , sexual dimorphism
Publications:
Resistance to high-fat-diet-induced obesity and sexual dimorphism in the metabolic responses of transgenic mice with moderate uncoupling protein 3 overexpression in glycolytic skeletal muscles. Tiraby C Tavernier G Capel F Mairal A Crampes F Rami J Pujol C Boutin JA Langin D. Diabetologia 2007 50 : 2190-2199. Mice overexpresing human uncoupling protein-3 in skeletal muscle are hyperphagic and lean. Clapham JC Arch JRS Chapman H et al. Nature 2000 406 : 415-418. Effect of the presence absence and overexpression of uncoupling protein-3 on adiposity and fuel metabolism in congenic mice. Costford SR Chaudry SN Salkhordeh M and ME Harper. Am. J. Physiol. Endocrinol. Metab. 2006 290 : E1304-E1312.

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