Rat mesothelial cell line F1-2

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a female rat (Fischer F344) after 306 days of induction with crocidolite (blue asbestos) injected intraperitoneously in 2008.
Classified as epithelioid, however, both morphology and marker levels correspond to an intermediary state (PNint subgroup) between epithelioid and sarcomatoid groups of cell lines.
With F5-2, these two cell lines differ from the eight other preneoplastic epithelioid cell lines of the biocollection (Sbnl and PNep subgroups) by their highest levels of fibronectin 1 (see graph on Fig. S1 in supplementary material of reference 2 below).
The complete proteomic analysis data of the biocollection of cell lines has been deposited on the PRIDE database website (http://www.ebi.ac.uk/pride), part of the ProteomeXchange Consortium, with the dataset identifier PXD060521.

Interest / Relevance: - Academic Research: identification of markers of neoplastic / transformed mesothelial cells, analyses of chromosomal damage induced by asbestos, studies of the mechanism of asbestos-induced oncogenesis, implication of fibrosis and inflammation.

- Industry research: antibody production, screening of new anticancer agents, identification of factors that increase mesothelial proliferation and migration.

- Therapeutic: development of epigenetic drugs, design of cancer vaccines, regenerative medicine applications.

Keywords: mesothelial cells, tumor marker, tumor invasion process, malignant transformation, proliferation rate

Scientist's name: M Daniel L. POULIQUEN

Publications:

1- Oncotarget (2016) 7, 34664-34687, doi: 10.18632/oncotarget.8970

2- Cancers (2020) 12, 939, doi: 10.3390/cancers12040939

Reference:

RT00409