Rat mesothelial cell line F1-0 (includes F1-0e and F1-0f subtypes)

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a female rat (Fischer F344), after 277 days of induction with crocidolite (blue asbestos) injected intraperitoneously in 2008.
This cell line was subdivided into F1-0e and F1-0f after 3 passages in culture. The two subtypes slightly differed both on morphological aspects (see photographs below at passage 7) and levels of markers.
Belongs to the epithelioid group and "Sbnl" (subnormal) subgroup (see publications 1 and 2 below for more details).
The complete proteomic analysis data of the biocollection of cell lines has been deposited on the PRIDE database website (http://www.ebi.ac.uk/pride), part of the ProteomeXchange Consortium, with the dataset identifier PXD060521.

Interest / Relevance: - Academic Research: identification of markers of neoplastic / transformed mesothelial cells, analyses of chromosomal damage induced by asbestos, studies of the mechanism of asbestos-induced oncogenesis, implication of fibrosis and inflammation.

- Industry research: antibody production, screening of new anticancer agents, identification of factors that increase mesothelial proliferation and migration.

- Therapeutic: development of epigenetic drugs, design of cancer vaccines, regenerative medicine applications.
Keywords: mesothelial cells, epithelioid, tumor marker, tumor invasion process, malignant transformation, proliferation rate
Scientist's name: M Daniel L. POULIQUEN
Publications:
1- Oncotarget (2016) 7, 34664-34687, doi: 10.18632/oncotarget.8970

2- Cancers (2020) 12, 939, doi: 10.3390/cancers12040939

Reference:

RT00420

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Organism: Rat
Tissue: Mesothelium (peritoneal cavity)
Morphology: Epithelioid
Passage Number: F1-0Ep: passages 4 and 7, F1-0Fib: passage 6
Culture Medium: RPMI 1640 supplemented with 10% FCS and 2mM L-Glutamine
Growth Properties: In 6-well plates (300,000 cells/well): averaged doubling times 45h and 38h, saturation density 110,000 and 130,000 cells / square cm, for F1-0e and F1-0f, respectively..
Karyotype: not determined
Rare disease: No
Last update: 27/02/2025

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