Gfp-tagged mucin-reporter mouse model

To follow the Muc5b gel-forming mucin production, we tagged with a GFP variant the corresponding gene. This reporter mouse allows to follow goblet cell number producing the mucin as well as the mucin production in tissues. Mating with Cre mouse deletes the last exon of Muc5b-GFP leading to mice deficient for Muc5b.

Keywords: mucus, mucin, eye, vagina, middle hear, endo- ectovervix, salivary gland, trachea, gallbladder, main bronchi, nose, olfactory epithelium

Publications:

Lacroix G, Gouyer V, Gottrand F, Desseyn JL : A porous cervical mucus plug leads to preterm birth induced by experimental vaginal infection in mice
iScience. 25(7): 104526. doi: 10.1016/j.isci.2022.104526

Amini SE, Gouyer V, Portal C, Gottrand F, Desseyn JL : Muc5b is mainly expressed and sialylated in the nasal olfactory epithelium whereas Muc5ac is exclusively expressed and fucosylated in the nasal respiratory epithelium.
Histochem. Cell Biol. 152(2): 167-174. doi: 10.1007/s00418-019-01785-5

Portal C, Gouyer V, Magnien M, Plet S, Gottrand F, Desseyn JL : In vivo imaging of the Muc5b gel-forming mucin.
Sci. Rep. 7:44591. doi: 10.1038/srep44591.

Portal C, Gouyer V, Gottrand F, Desseyn JL : Preclinical mouse model to monitor live Muc5b-producing conjunctival goblet cell density under pharmacological treatments.

Reference:

RT00480

Type of research tool: Laboratory Animals

Uses(1): Academic Research

Uses(2): Industry Research (screening, tox.studies, bioreactor, ...)

Uses(3): Therapeutic

Inserm Transfert
Research Tools

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Species: mousenull

Genotype: hétézozygous and homozygous knockin for the mucin gene

Strain: 129 backcrossed in C57BL/6

Genetic Background: mix 129 and C57BL/6

Applications: toxicity, drug effect

Rare disease: No

Last update: 18/11/2024

Gfp-tagged mucin-reporter mouse model

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