Mouse t lymphoma cell line – ko99l

The murine primary KO99L cell line was established by cultivating tumoral thymocytes from a tPTEN-/- mouse. Mice carrying a T cell-specific deletion of PTEN develop invasive T-cell lymphomas and die within 10-15 weeks. KO99L cells display an abnormal constitutive activation of the PI3K/Akt/mTOR pathway that supports their enhanced proliferation and deregulated metabolism, as well as their resistance to cell death. This cell line also displayed an amplification of c-Myc .

Interest / Relevance: Representative of T-cell Acute Lymphoblastic Leukemia/Lymphoblastic Lymphoma (T-ALL/LL)
Model of mouse T lymphoma cells with constitutive activation of PI3/Akt/mTor et c-myc upregulation.
In vitro screening of antileukemics drugs-Combo experiments
Tumorigenic in nude mice (subcutaneous injection).

Keywords: T-ALL/LL , PI3K/Akt/mTor , c-myc

Scientist's name: Véronique IMBERT
Jean-François PEYRON

Publications:

Rosilio, C., Nebout, M., Imbert, V., Griessinger, E., Neffati, Z., Benadiba, J., Hagenbeek, T., Spits, H., Reverso, J., Ambrosetti, D., Michiels, J. F., Bailly-Maitre, B., Endou, H., Wempe, M. F., and Peyron, J. F. (2015). L-type amino-acid transporter 1 (LAT1): a therapeutic target supporting growth and survival of T-cell lymphoblastic lymphoma/T-cell acute lymphoblastic leukemia. Leukemia 29, 1253-1266.

Rosilio, C., Lounnas, N., Nebout, M., Imbert, V., Hagenbeek, T., Spits, H., Asnafi, V., Pontier-Bres, R., Reverso, J., Michiels, J. F., Sahra, I. B., Bost, F., and Peyron, J. F. (2013). The metabolic perturbators metformin, phenformin and AICAR interfere with the growth and survival of murine PTEN-deficient T cell lymphomas and human T-ALL/T-LL cancer cells. Cancer Lett 336, 114-126.

Lounnas, N., Rosilio, C., Nebout, M., Mary, D., Griessinger, E., Neffati, Z., Chiche, J., Spits, H., Hagenbeek, T. J., Asnafi, V., Poulsen, S. A., Supuran, C. T., Peyron, J. F., and Imbert, V. (2013). Pharmacological inhibition of carbonic anhydrase XII interferes with cell proliferation and induces cell apoptosis in T-cell lymphomas. Cancer Lett 333, 76-88.

Reference:

RT00493