Hg5ln gal4-hppar gamma cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the (GAL4RE)5-betaGlobin-Luciferase-SV40-Neomycin plasmid.
HG5LN GAL4-hPPAR gamma cells were generated by transfection of HG5LN cells with the plasmid pSG5-GAL4-human PPAR gamma-puromycin.

The plasmid (GAL4RE)5-betaGlob-Luc-SVNeo contains a luciferase gene driven by a pentamer of yeast activator GAL4 binding sites in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter.
The pSG5-puro plasmid pSG5-human GAL4-hPPAR gamma-puromycin enables to express the DNA binding domain of the yeast activator GAL4 (GAL4 DBD) followed by the ligand binding domain of the human peroxisome proliferator activated receptor receptor gamma (hPPAR gamma LBD). Puromycin N-acetyl transferase selection marker expression confers resistance to puromycin.

Interest / Relevance: These cells enable to measure the hPPAR gamma (full and partial agonism, antagonism) of pharmaceuticals (metabolic diseases), environmental compounds (bisphenols).
These cells enable to measure the hPPAR gamma activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.
Rosiglitazone EC50 18 nM

Keywords: hPPAR gamma, luciferase, pharmaceutical and environmental chemicals

Scientist's name: Patrick BALAGUER
Patrick Balaguer

Publications:

Seimandi M, Lemaire G, Pillon A, Perrin A, Carlavan I, Voegel JJ, Vignon F, Nicolas JC, Balaguer P. Differential responses of PPARalpha, PPARdelta, and PPARgamma reporter cell lines to selective PPAR synthetic ligands. Anal Biochem. 2005, 344: 8-15.
Riu A, le Maire A, Grimaldi M, Boulahtouf A, Audebert M, Hillenweck A, Bourguet W, Balaguer P, Zalko D, Characterization of novel ligands of ER alpha, ER beta and PPAR gamma : the case of halogenated BPA and their conjugation metabolites. Toxicol Sci. 2011 , 122 : 372-82.
Riu A, Grimaldi M, le Maire A, Bey G, Phillips K, Boulahtouf A, Perdu E, Zalko D, Bourguet W, Balaguer P. Peroxysome proliferator-activated receptor gamma is a target for halogenated analogues of bisphenol-A. Environ Health Perspect. 2011 , 119 : 1227-32.
Garoche C, Boulahtouf A, Grimaldi M, Toporova L, den Broeder MJ, Legler J, Bourguet W, Balaguer P*. Interspecies differences in activation of Peroxysome proliferator-activated receptor ? by pharmaceutical and environmental chemicals. Environ Sci Technol 2021 doi 10.1021/acs.est.1c04318

Reference:

RT00559

Type of research tool: Cell Lines

Therapy area(1): Oncology

Uses(1): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

E-mail

Culture Medium: DMEM-F12, 5% FCS, 1% gentamycin, G418 1 mg/ml, pyuromycin 0,5 microgram/ml

Rare disease: No

Last update: 11/09/2024

Hg5ln gal4-hppar gamma cell line

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