HELN were generated by transfection of cervical cancer HeLa cells with the ERE-betaGlobin-Luciferase-SV40-Neomycin plasmid.
The plasmid ERE-betaGlob-Luc-SVNeo contains a luciferase gene driven by estrogen receptor responsive element (ERE) in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter.