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Cpt1 expressing yeast strains

Saccharomyces cerevisiae expressing under the GAL promoter either the wild-type rat liver CPT1, mutated rat liver CPT1 or chimeric CPT. CPT1A activity is inhibited by malonyl-CoA, its allosteric inhibitor.

Interest / Relevance: Allow to have isolated mitochondria with wild-type rat liver CPT1 (active and sensitive to malonyl-CoA inhibition) or mutated CPT1 (for example, active but insensitive to malonyl-CoA inhibition). These mitochondria could be used as biological material for screening chemical molecules able to modulate CPT1 activity and malonyl-CoA sensitivity.

Scientist's name: Carina PRIP-BUUS

Publications:

Cohen I et al. (1998) J. Biol. Chem. 273 : 29896-29904.
Cohen I et al. (2001) J. Biol. Chem. 276 : 5403-5411.
Faye A et al. (2007) J. Biol. Chem. 282 : 26908-16.
Faye A et al. (2005) Biochem. J. 387 : 67-76.

Reference:

RT00005

Type of research tool: Cell Lines

Uses(1): Academic Research

Uses(2): Proof of concept in vitro

Uses(3): Proof of concept in vivo

Uses(4): Industry Research (screening, tox.studies, bioreactor, ...)

Uses(5): Therapeutic

Antibody applications: In vivo studies

Inserm Transfert
Research Tools

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Organism: Saccharomyces cerevisiae

Tissue: Unicellular

Morphology: Not relevant

Passage Number: Not relevant

Subculture Routine: Not relevant

Culture Medium: Minimal selective medium. Induction of protein expression by addition of galactose

Growth Properties: Not relevant

Karyotype: Not relevant

Rare disease: No

Last update: 11/09/2024

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