Ex vivo culture system for screening compounds targeting leukaemia-initiating cells

A novel, ex vivo niche-based hypoxic culture system for maintaining Leukaemia Initiating Cells (LICs) from patients and an associated method to quantify their dynamics.

Interest / Relevance: The system and method permit efficient screening of compounds targeting leukaemia-initiating cells more quickly and affordable than the xenotransplantation assay in immunodeficient NSG mice. The approach also allows the analysis of effects/toxicity of compounds on normal hematopoïetic stem cells.

Keywords: Leukaemia Initiating Cells (LICs) , Acute Myeloid Leukaemia (AML) , Stem cells Niche , Self-renewal

Scientist's name: Emmanuel GRIESSINGER
Jean-François PEYRON

Publications:

Griessinger E, et al. 2014. A Niche-Like Culture System Allowing the Maintenance of Primary Human Acute Myeloid Leukemia-Initiating Cells: A New Tool to Decipher Their Chemoresistance and Self-Renewal Mechanisms. Stem cells Translational Medicine. 3, 520-9.

Reference:

RT00483

Type of research tool: Know How

Therapy area(1): Oncology

Uses(1): Industry Research (screening, tox.studies, bioreactor, ...)

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Research Tools

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Accessible for: Fee-for-service research, Collaboration, Training / Know-How License

Equipment available: This method requires a flow cytometer with at least two, at best three lasers, an hypoxia chamber or incubator and a Class II biosafety cabinet for handling human primary samples.

Rare disease: No

Last update: 11/09/2024

Ex vivo culture system for screening compounds targeting leukaemia-initiating cells

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