Hg5ln gal4-hppar beta/delta cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the (GAL4RE)5-betaGlobin-Luciferase-SV40-Neomycin plasmid. rnHG5LN GAL4-hPPAR beta/delta cells were generated by transfection of HG5LN cells with the plasmid pSG5-GAL4-human PPAR beta/delta-puromycin.

The plasmid (GAL4RE)5-betaGlob-Luc-SVNeo contains a luciferase gene driven by a pentamer of yeast activator GAL4 binding sites in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. rnThe pSG5-puro plasmid pSG5-human GAL4-hPPAR beta/delta -puromycin enables to express the DNA binding domain of the yeast activator GAL4 (GAL4 DBD) followed by the ligand binding domain of the human peroxisome proliferator activated receptor receptor beta/delta (hPPAR beta/delta LBD). Puromycin N-acetyl transferase selection marker expression confers resistance to puromycin.rn

Interest / Relevance: These cells enable to measure the hPPAR beta/delta activity (full and partial agonism, antagonism) of pharmaceuticals (metabolic diseases), environmental compounds (bisphenols). rnThese cells enable to measure the hPPAR beta/delta activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.rnGW501516 EC50 1.8 nMrn
Keywords: hPPAR beta/delta, luciferase, pharmaceutical and environmental chemicals
Scientist's name: Patrick BALAGUER
Patrick Balaguer
Seimandi M Lemaire G Pillon A Perrin A Carlavan I Voegel JJ Vignon F Nicolas JC Balaguer P. Differential responses of PPARalpha PPARdelta and PPARgamma reporter cell lines to selective PPAR synthetic ligands. Anal Biochem. 2005 344: 8-15.

You might also be interested in