Heln her alpha cell line

HELN were generated by transfection of cervical cancer HeLa cells with the ERE-betaGlobin-Luciferase-SV40-Neomycin plasmid.
HELN hER alpha cells were generated by transfection of HELN cells with the plasmid pSG5-human ERalpha-puromycin.

Interest / Relevance: These cells enable to measure the hER alpha estrogenic activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics).
These cells enable to measure the hER alpha estrogenic activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.
Estradiol EC50 17 pM

Keywords: hER alpha, luciferase, pharmaceutical and environmental estrogens

Scientist's name: Patrick BALAGUER
Patrick BALAGUER

Publications:

Escande A, Pillon A, Servant N, Cravedi JP, Larrea F, Muhn P, Nicolas JC, Cavailles V, Balaguer P. Evaluation of ligand selectivity using reporter cell lines stably expressing estrogen receptor alpha or beta. Biochem Pharmacol. 2006, 71: 1459-69.
Delfosse V, Grimaldi M, Pons JL, Boulahtouf A, le Maire A, Cavailles V, Labesse G, Bourguet W, Balaguer P. Structural and mechanistic insights into bisphenols action provide guidelines for risk assessment and discovery of bisphenol A substitutes. Proc Natl Acad Sci U S A. 2012 , 109 : 14930-5.
Delfosse V, Grimaldi M, Cavaillès V, Balaguer P, Bourguet W. Structural and Functional Profiling of Estrogen Receptors Environmental Ligands. Environ Health Perspect. 2014, 122: 1306-13.

Reference:

RT00316

Type of research tool: Cell Lines

Therapy area(1): Oncology

Therapy area(3): Women health

Uses(1): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

E-mail

Organism: human

Tissue: cervix

Culture Medium: DMEM red phenol free, t 5% stripped serum , puromycine 0,5 microg/ml, geniticine 1 mg/ml

Rare disease: No

Last update: 11/09/2024

Heln her alpha cell line

You might also be interested in

Transgenic mouse model for myotonic dystrophy:

Rat anti-mouse cd26 (mouse dipeptidyl peptidase

Gfp-tagged mucin-reporter mouse model

Rbl-2h3 rat mast cell line : in vitro

Mouse anti-histone h1zero (clone 27e8e10)

Mouse anti-histone h1zero (clone 34b10h4)

Mouse anti mouse ccl5 monoclonal antibody (clone

Human ferroportin polyclonal antibodies

Technique to isolate live renal proximal tubular

Primary cultures of mice proximal tubular cells

Hg5ln gal4-hppar gamma cell line

Cpt1 expressing yeast strains