Heln er beta cell line

HELN were generated by transfection of cervical cancer HeLa cells with the ERE-betaGlobin-Luciferase-SV40-Neomycin plasmid.
HELN hER beta cells were generated by transfection of HELN cells with the plasmid pSG5-human ERbeta-puromycin.

Lignée cellulaire de cancer du col de l?utérus HeLa (ER négative) transfectée premièrement par le plasmide de la luciférase firefly sous le contrôle d?un élément de réponse au récepteur des oestrogènes ERE, secondairement par un plasmide d?expression du récepteur des oestrogènes humain beta

Interest / Relevance: These cells enable to measure the hER beta estrogenic activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics).
These cells enable to measure the hER beta estrogenic activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.
Estradiol EC50 68 pM

Keywords: hER beta, luciferase, pharmaceutical and environmental estrogens

Scientist's name: Patrick BALAGUER
Patrick Balaguer

Publications:

Escande A, Pillon A, Servant N, Cravedi JP, Larrea F, Muhn P, Nicolas JC, Cavailles V, Balaguer P. Evaluation of ligand selectivity using reporter cell lines stably expressing estrogen receptor alpha or beta. Biochem Pharmacol. 2006, 71: 1459-69.
Delfosse V, Grimaldi M, Pons JL, Boulahtouf A, le Maire A, Cavailles V, Labesse G, Bourguet W, Balaguer P. Structural and mechanistic insights into bisphenols action provide guidelines for risk assessment and discovery of bisphenol A substitutes. Proc Natl Acad Sci U S A. 2012 , 109 : 14930-5.
Delfosse V, Grimaldi M, Cavaillès V, Balaguer P, Bourguet W. Structural and Functional Profiling of Estrogen Receptors Environmental Ligands. Environ Health Perspect. 2014, 122: 1306-13.

Reference:

RT00317

Type of research tool: Cell Lines

Therapy area(1): Oncology

Therapy area(3): Women health

Uses(1): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

E-mail

Organism: human

Tissue: cervix

Rare disease: No

Last update: 11/09/2024

Heln er beta cell line

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