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Rat mesothelial cell line m3-1

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a male rat (Fischer F344) after 265 days of induction with crocidolite (blue asbestos) injected intraperitoneously.
Interest / Relevance: - Academic Research: identification of markers of neoplastic / transformed mesothelial cells, analyses of chromosomal damage induced by asbestos, studies of the mechanism of asbestos-induced oncogenesis, implication of fibrosis and inflammation.
- Industry research: antibody production, screening of new anticancer agents, identification of factors that increase mesothelial proliferation and migration.
- Therapeutic: development of epigenetic drugs, design of cancer vaccines, regenerative medicine applications.
Cellular and Molecular Biology, Immunology.
Keywords: mesothelial cells , tumor marker , tumor invasion process , malignant transformation , proliferation rate
Scientist's name: Daniel POULIQUEN

Reference:

RT00428

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Inserm Transfert
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Organism: Rat
Tissue: Mesothelium (peritoneal cavity)
Morphology: Epithelioid
Passage Number: 5
Culture Medium: RPMI 1640 supplemented with 10% FCS and 2mM L-Glutamine
Growth Properties: In 6-well plates (300,000 cells/well): Doubling time 48-72h. Saturation density 100,000-200,000 cells / sq-cm.
Karyotype: under investigation
Rare disease: No
Last update: 11/09/2024

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