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Ms-5-omi/htra2-mcherry cells

Murine stromal MS-5 cells were transduced with a retroviral pBabe vector to stably express the Omi/HTRA2-mCherry protein. OMI/HTRA2 is a marker of intact polarized mitochondria that is rapidly degraded following mitochondrial outer membrane permeabilization (MOMP).

Interest / Relevance: Feeder cells for long-term culture of normal and Acute Leukemia Myeloid cells
mCherry fluorescence is a read-out of mitochondria integrity.
MS5-Omi/HTRA2-mCherry cells can be used to study mitochondrial transfer.
Mouse Stromal cells

Keywords: Stromal cells , LTC-IC , Omi-mCherry , Mitochondrial transfer

Scientist's name: Véronique IMBERT
Emmanuel GRIESSINGER, Jean-François PEYRON

Reference:

RT00494

Type of research tool: Cell Lines

Therapy area(1): Oncology

Therapy area(2): Others

Uses(1): Academic Research

Uses(2): Proof of concept in vitro

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Organism: Mouse

Tissue: Stromal cells

Morphology: Adherent cells

Subculture Routine: Split confluent culture 1:3 to 1:10 every third day using trypsin/EDTA

Culture Medium: IMDM supplemented with 2mM L-Glutamine 25mM Hepes 10%SVF Penicillin Streptomycin

Rare disease:

Last update: 05/06/2023

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