Meln (her alpha) cell line

MELN were generated by transfection of breasr cancer MCF-7 cells with the ERE-betaGlobin-Luciferase-SV40-Neomycin plasmid.
The plasmid ERE-betaGlob-Luc-SVNeo contains a luciferase gene driven by estrogen receptor responsive element (ERE) in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter.

Interest / Relevance: These cells enable to measure the hER alpha estrogenic activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics).
These cells enable to measure the hER alpha estrogenic activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.

Keywords: Estrogen receptor alpha, luciferase, pharmaceutical and environmental estrogens

Scientist's name: Patrick BALAGUER
Patrick Balaguer

Publications:

Balaguer P, Boussioux AM, Demirpence E, Nicolas JC. Reporter cell lines are useful tools for monitoring biological activity of nuclear receptor ligands. Luminescence. 2001, 16:1 53-8.
Fenet H, Gomez E, Pillon A, Rosain D, Nicolas JC, Casellas C, Balaguer P. Estrogenic activity in water and sediments of a French river: contribution of alkylphenols. Arch Environ Contam Toxicol. 2003, 44: 1-6.

Reference:

RT00583

Type of research tool: Cell Lines

Therapy area(1): Oncology

Uses(1): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

E-mail

Rare disease: No

Last update: 11/09/2024

Meln (her alpha) cell line

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