HRLN were generated by transfection of cervical cancer HeLa cells with the (RARE)3-TK-Luciferase-SV40-Neomycin plasmid.
The plasmid (RARE)3-TK-Luc-SVNeo contains a luciferase gene driven by three retinoid receptor responsive element (RARE) in front of thymidine kinase promoter and a neomycin phosphotransferase gene under the control of SV40 promoter.