Back to results

Hahlh (hahr) cell line

HAHLH were generated by transfection of cervical cancer HeLa cells with the XRE(TnGCGTG)3-TATA-Luciferase-SV40-Hygromycin plasmid.
The plasmid XRE(TnGCGTG)3-TATA -Luc-SVHygro contains a luciferase gene driven by three AhR responsive elements and a hygromycin gene under the control of SV40 promoter.
Interest / Relevance: These cells enable to measure the hAhR activity (full and partial agonism, antagonism) of pharmaceuticals, environmental compounds (HAPs, PCBs, dioxins).
These cells enable to measure the hAhR activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.

Dioxin EC50 5 nM
Keywords: hAhR, luciferase, pharmaceutical and environmental hAhR ligands
Scientist's name: Patrick BALAGUER
Patrick Balaguer
Publications:
Gruszczyk J, Grandvuillemin L, Lai-Kee-Him J, Paloni M, Christos G, Savva CG, Germain P, Grimaldi M, Boulahtouf A, Kwong HK, Bous J, Ancelin A, Bechara C, Barducci A, Balaguer P, Bourguet W. Cryo-EM structure of the agonist-bound Hsp90-XAP2-AHR cytosolic complex. Nat Commun 2022 Nov 16,13(1):7010.

Reference:

RT00618

Business Developper
contact
Inserm Transfert
Research Tools
Rare disease: No
Last update: 11/09/2024

You might also be interested in

RT00399

Transgenic mouse model for myotonic dystrophy:

This transgenic mice model contains large human genomic DNA sequence carrying the DMPK gene with […]

Read more
RT00344

Rat anti-mouse cd26 (mouse dipeptidyl peptidase

Rat monoclonal antibody recognizing the cell surface antigen DPP IV (CD26) involved in T cell […]

Read more
RT00480

Gfp-tagged mucin-reporter mouse model

To follow the Muc5b gel-forming mucin production, we tagged with a GFP variant the corresponding […]

Read more
RT00394

Rbl-2h3 rat mast cell line : in vitro

Rat mast cell line (RBL-2H3) transfected with the cDNAs encoding the three subunits (alpha, beta, […]

Read more
RT00286

Mouse anti-histone h1zero (clone 27e8e10)

[…]

Read more
RT00287

Mouse anti-histone h1zero (clone 34b10h4)

[…]

Read more
RT00320

Mouse anti mouse ccl5 monoclonal antibody (clone

Mouse monoclonal antibody directed against mouse CCL5.
Antibody was produced by immunising CCL5 […]

Read more
RT00484

Mouse anti-human protein s (pros1) mabs

These mouse monoclonal antibodies are directed against human PROS1, an anti-coagulation factor. They […]

Read more
RT00469

Human ferroportin polyclonal antibodies

Rabbit Polyclonal antibodies raised against 3 differents portions of the iron exporter Human […]

Read more
RT00072

Mouse anti-schwann cell (clone 224-58)

Positive staining observed on human, rabbit, rat and mouse peripheral nerve sections or Schwann cell […]

Read more
RT00488

Technique to isolate live renal proximal tubular

We isolate renal proximal tubular cells using the proximal tubular marker Prominin-1.
We use a […]

Read more
RT00486

Primary cultures of mice proximal tubular cells

Proximal tubular cells are isolated by magnetic sorting coupled with microbeads-conjugated […]

Read more