Heln cell line

HELN were generated by transfection of cervical cancer HeLa cells with the ERE-betaGlobin-Luciferase-SV40-Neomycin plasmid.

The plasmid ERE-betaGlob-Luc-SVNeo contains a luciferase gene driven by estrogen receptor responsive element (ERE) in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter.

Interest / Relevance: These cells are a negative control cell line for measurement of estrogenic activity.

Keywords: Estrogen receptors, luciferase, pharmaceutical and environmental estrogens

Scientist's name: Patrick BALAGUER
Patrick Balaguer

Publications:

Escande A, Pillon A, Servant N, Cravedi JP, Larrea F, Muhn P, Nicolas JC, Cavailles V, Balaguer P. Evaluation of ligand selectivity using reporter cell lines stably expressing estrogen receptor alpha or beta. Biochem Pharmacol. 2006, 71: 1459-69.
Delfosse V, Grimaldi M, Pons JL, Boulahtouf A, le Maire A, Cavailles V, Labesse G, Bourguet W, Balaguer P. Structural and mechanistic insights into bisphenols action provide guidelines for risk assessment and discovery of bisphenol A substitutes. Proc Natl Acad Sci U S A. 2012 , 109 : 14930-5.
Delfosse V, Grimaldi M, Cavaillès V, Balaguer P, Bourguet W. Structural and Functional Profiling of Estrogen Receptors Environmental Ligands. Environ Health Perspect. 2014, 122: 1306-13.

Reference:

RT00619

Type of research tool: Cell Lines

Therapy area(1): Oncology

Uses(1): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

E-mail

Rare disease: No

Last update: 11/09/2024

Heln cell line

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