Using BRET technology, we designed a method to measure histone acetylation in living cells. Cells are transfected with a vector coding for a bromodomain fused to renilla luciferase (Rluc-BrD) and with a vector coding for histone H3 fused to yellow fluorescent protein (YFP). In the presence of HDACi, Rluc-BrD and YFP-histone H3 interact and generate an increase of BRET signal.