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Three-dimensional multicellular spheroid cultures

When culture in suspension many mammalian cell types can aggregate into 3D-spheroids. These multicellular cultures mimic real tissues or tumours and are useful models for therapeutic screening.

Interest / Relevance: Intermediate assay between in cellulo and in vivo cultures; in some extent predictive for in vivo results.
Test useful for:
- Proliferation assays
- Cell-cell interaction analysis
- Drug availability (clearance, half-life, ...)
- Gene profiling

Avantages: long term cultures, daily tracing of spheroid size.

Keywords: proliferation , tumour , cell toxicity , 3d-cultures , cell-cell interactions

Scientist's name: Annie MOLLA

Publications:

Hoang TM Favier B Valette A Barette C Nguyen CH Lafanechère L Grierson DS Dimitrov S Molla A.
Benzo[e]pyridoindoles novel inhibitors of the aurora kinases.
Cell Cycle. 2009 Mar 1;8(5):765-72.

Reference:

RT00396

Type of research tool: Know How

Therapy area(1): Oncology

Therapy area(2): Others

Uses(1): Academic Research

Uses(2): Proof of concept in vitro

Uses(3): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

E-mail

Accessible for: Fee-for-service research, Collaboration, Training / Know-How License

Equipment available: Cell culture facilities/ microscopy

Samples/Models available: Many cell types are eligible.Requirement: aggregating cells.Examples: NSCLC H358, HCT-116, ...

Rare disease: No

Last update: 11/09/2024

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