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Stable human prolactin receptor ba/f3 cell

Ba/F3 cells are mouse pro-B lymphocytes that are dependent on IL-3 for survival/growth. We stably transfeted them with expression vector encoding the long isoform of the human prolactin receptor. Their survival/growth can now be shifted to prolactin in the absence of IL-3.

Interest / Relevance: It is used to test any ligand of the prolactin receptor, based on cell proliferation and survival. It can also be used for signaling studies of the prolactin receptor. It can also be usd for evaluating the amount of prolactin receptor agonist in a fluid.

Keywords: prolactin , prolactin receptor , luciferase , agonists , antagonists

Scientist's name: Vincent GOFFIN

Reference:

RT00003

Type of research tool: Cell Lines

Therapy area(1): Others

Uses(1): Academic Research

Uses(2): Industry Research (screening, tox.studies, bioreactor, ...)

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Organism: Mouse

Tissue: Pro-B lymphocytes

Morphology: Suspension, round cells

Culture Medium: Classical RPMI with additives

Growth Properties: Passage every other day (do not like being too diluted or confluent)

Rare disease:

Last update: 05/06/2023

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