Rbl-2h3 rat mast cell line : in vitro degranulation assay for evaluation of ige-reactivity towards environmental allergens in allergic patient serum

Rat mast cell line (RBL-2H3) transfected with the cDNAs encoding the three subunits (alpha, beta, gamma) of the human high affinity IgE receptor. The transfected cell line binds human IgE, which does not bind to rodent receptor. It enablesto perform degranulation assays with serum from allergic patients for its reactivity towards environmental allergens.

Interest / Relevance: 1. Evaluation of patient serum for reactivity towards environmental allergen.
2. Evaluation of adverse "allergic" effects in clinical studies or trials.
Keywords: allergy , mast cell , human IgE
Scientist's name: Ulrich BLANK

Reference:

RT00394

Business Developper
contact
Ulrich BLANK
Inserm Transfert
Research Tools
Organism: Rat (Lewis)
Tissue: Mucosal mast cell line
Morphology: NA
Passage Number: Unknown
Subculture Routine: NA
Culture Medium: DMEM with 10% fetal calf serum
Growth Properties: Adherent
Karyotype: NA, In vitro human mast cell degranulation assay
Rare disease:
Last update: 15/06/2023

You might also be interested in

Contact

Inserm Transfert

Research tool licensing team

researchtools@inserm-transfert.fr