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Rat mesothelial cell line m2-2

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a male rat (Fischer F344) after 270 days of induction with crocidolite (blue asbestos) injected intraperitoneously.

Interest / Relevance: - Academic Research: identification of markers of neoplastic / transformed mesothelial cells, analyses of chromosomal damage induced by asbestos, studies of the mechanism of asbestos-induced oncogenesis, implication of fibrosis and inflammation.
- Industry research: antibody production, screening of new anticancer agents, identification of factors that increase mesothelial proliferation and migration.
- Therapeutic: development of epigenetic drugs, design of cancer vaccines, regenerative medicine applications.
Cellular and Molecular Biology, Immunology.

Keywords: mesothelial cells , tumor marker , tumor invasion process , malignant transformation , proliferation rate

Scientist's name: Daniel POULIQUEN

Reference:

RT00426

Type of research tool: Cell Lines

Therapy area(1): Oncology

Therapy area(2): Immunology

Therapy area(3): Others

Therapy area(4): Infectious Diseases

Uses(1): Academic Research

Uses(2): Proof of concept in vitro

Uses(3): Industry Research (screening, tox.studies, bioreactor, ...)

Uses(4): Diagnostic

Uses(5): Therapeutic

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Organism: Rat

Tissue: Mesothelium (peritoneal cavity)

Morphology: Sarcomatoid

Passage Number: 4, 5

Culture Medium: RPMI 1640 supplemented with 10% FCS and 2mM L-Glutamine

Growth Properties: In 6-well plates (300,000 cells/well): Doubling time: 24-48h. Saturation density: 100,000-200,000 cells / sq-cm.

Karyotype: under investigation

Rare disease: No

Last update: 11/09/2024

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