Mouse t lymphoma cell line expressing luciferase – ko99l/luc

The primary KO99L cell line was established by cultivating tumoral thymocytes from a tPTEN-/- mouse. KO99L cells were transduced with a pLenti-III-luc23 construct to stably express luciferase.
Several clones are available.

Interest / Relevance: This cell line is suitale for in vitro and in vivo analyses of tumor growth by bioluminescence.
Tumorigenic in nude mice.

Keywords: T ALL/LL , Imaging , Tumor growth , Bioluminescence

Scientist's name: Véronique IMBERT
Jean-François PEYRON

Publications:

Rosilio, C., Lounnas, N., Nebout, M., Imbert, V., Hagenbeek, T., Spits, H., Asnafi, V., Pontier-Bres, R., Reverso, J., Michiels, J. F., Sahra, I. B., Bost, F., and Peyron, J. F. (2013). The metabolic perturbators metformin, phenformin and AICAR interfere with the growth and survival of murine PTEN-deficient T cell lymphomas and human T-ALL/T-LL cancer cells. Cancer Lett 336, 114-126.

Rosilio, C., Nebout, M., Imbert, V., Griessinger, E., Neffati, Z., Benadiba, J., Hagenbeek, T., Spits, H., Reverso, J., Ambrosetti, D., Michiels, J. F., Bailly-Maitre, B., Endou, H., Wempe, M. F., and Peyron, J. F. (2015). L-type amino-acid transporter 1 (LAT1): a therapeutic target supporting growth and survival of T-cell lymphoblastic lymphoma/T-cell acute lymphoblastic leukemia. Leukemia 29, 1253-1266.

Reference:

RT00492