Back to results

Hahlh (hahr) cell line

HAHLH were generated by transfection of cervical cancer HeLa cells with the XRE(TnGCGTG)3-TATA-Luciferase-SV40-Hygromycin plasmid.
The plasmid XRE(TnGCGTG)3-TATA -Luc-SVHygro contains a luciferase gene driven by three AhR responsive elements and a hygromycin gene under the control of SV40 promoter.
Interest / Relevance: These cells enable to measure the hAhR activity (full and partial agonism, antagonism) of pharmaceuticals, environmental compounds (HAPs, PCBs, dioxins).
These cells enable to measure the hAhR activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.

Dioxin EC50 5 nM
Keywords: hAhR, luciferase, pharmaceutical and environmental hAhR ligands
Scientist's name: Patrick BALAGUER
Patrick Balaguer
Publications:
Gruszczyk J Grandvuillemin L Lai-Kee-Him J Paloni M Christos G Savva CG Germain P Grimaldi M Boulahtouf A Kwong HK Bous J Ancelin A Bechara C Barducci A Balaguer P Bourguet W. Cryo-EM structure of the agonist-bound Hsp90-XAP2-AHR cytosolic complex. Nat Commun 2022 Nov 1613(1):7010.

Reference:

RT00618

Business Developper
contact
Inserm Transfert
Research Tools
Rare disease: No
Last update: 11/09/2024

You might also be interested in

RT00559

Hg5ln gal4-hppar gamma cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the […]

Read more
RT00433

Conditional bioluminescent tmp3-alk cell line

Overexpression and activation of TMP3-ALK tyrosine kinase fusion protein is a causal oncogenic event […]

Read more
RT00005

Cpt1 expressing yeast strains

Saccharomyces cerevisiae expressing under the GAL promoter either the wild-type rat liver CPT1, […]

Read more
RT00618

Hahlh (hahr) cell line

HAHLH were generated by transfection of cervical cancer HeLa cells with the […]

Read more
RT00621

Hg5ln gal4-rtr beta cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the […]

Read more
RT00623

Hrln (hrar alpha) cell line

HRLN were generated by transfection of cervical cancer HeLa cells with the […]

Read more
RT00619

Heln cell line

HELN were generated by transfection of cervical cancer HeLa cells with the […]

Read more
RT00620

Heln hrar alpha cell line

HELN were generated by transfection of cervical cancer HeLa cells with the […]

Read more
RT00626

Ug5ln gal4-hmr cell line

UG5LN were generated by transfection of osteocarcinoma cancer U2OS cells with the […]

Read more
RT00624

Ualh cell line

UALH were generated by transfection of osteocarcinoma cancer U2OS cells with the […]

Read more
RT00569

Hg5ln gal4-hppar beta/delta cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the […]

Read more
RT00582

Palm cell line

PALM were generated by co-transfection of cervical cancer PC3 cells with the […]

Read more