Animal models of fibrosis

UUO: This model is induced in mice or rats by unilateral ureteral ligation (UUO) of the left ureter. The animals rapidly develop, within a few days, severe tubulo-interstitial renal fibrosis with an inflammatory cell infiltrate in the affected kidney.

SNX: This model consists at reducing the renal mass by 83%. This induces hyperfiltration and induces over a period of around 6 months significant fibrotic lesions in C57bl/6j mice and in 2 months in Swiss mice. In rats the model is somewhat faster as it induces renal fibrosis in 3 months.

Interest / Relevance: UUO: This is an ideal go or no-go model to test the potential anti-fibrotic activity of (new) compounds.

SNX: This is a more chronic model of the development of fibrosis somewhat closer to what is observed in humans than the UUO model.

Keywords: Chronic kidney disease , Fibrosis , In vivo drug evaluation

Scientist's name: Joost SCHANSTRA
Jean-Loup BASCANDS

Publications:

UUO: Schanstra J. P. Neau E. Drogoz P. Arevalo Gomez M. A. et al. In vivo bradykinin B2 receptor activation reduces renal fibrosis. J Clin Invest 2002.

UUO: Pradere J. P. Klein J. Gres S. Guigne C. et al. LPA1 receptor activation promotes renal interstitial fibrosis. J Am Soc Nephrol 2007

SNX: Schanstra J. P. Bachvarova M. Neau E. Bascands J. L. and Bachvarov D. Gene expression profiling in the remnant kidney model of wild type and kinin B1 and B2 receptor knockout mice. Kidney Int 2007

Reference:

RT00449